Qalabka Sifaynta ee Oligo sifaynta
Tilmaamid
| Looxyada ku habboon | 1, 3, 5, 8. |
| Sifaynta | shaandhaynta dharbaaxo, sifaynta nuugista |
| Tirada dekedaha la isku duro | 5, 6, 7, 8, 9, 10. |
| Noocyada saxanka ee ku habboon | saxan C18, saxan ceel qoto dheer, saxan synthetic (ku habboon inta badan taarikada synthetic), saxan microtiter |
| Module | dhidib keliya ama dhidibo laba geesood ah |
| Voltage | 220V |
| Dammaanad | 1 sano |
| Caadiyan | Waa la aqbalay |
Siyaabaha kala duwan ee Nadiifinta
1. Jel Electrophoresis Chromatography Nadiifinta
Isticmaal denaturing polyacrylamide jel electrophoresis chromatography si loo nadiifiyo.Wakiilka ilkaha guud ahaan waa 4M formamide ama 7M urea, fiirsashada acrylamide waa inta u dhaxaysa 5-15%, iyo saamiga methacrylamide inta badan waa inta u dhaxaysa 2-10%.
Ka dib electrophoresis, booska band nucleic acid ayaa loo baahan yahay in la go'aamiyo hoos shucaaca iftiinka ultraviolet, jel ay ku jiraan bartilmaameedka nucleic acid waa la gooyaa, nukleic acid waa la jejebiyey oo leach, ka dibna xal leaching ayaa xoogga saaray, macmalay, tiro iyo lyophilized.
2. DMT-On, HPLC Nadiifinta
Dooro qaabka DMT-On inta lagu guda jiro isku-dhafka, badeecada ceyriinka ah ayaa la xoojiyay oo lagu ururiyay heerkulka qolka si meesha looga saaro ammonia dheeraadka ah ka dib aminolysis-ka.
Kala soocida waxaa lagu sameeyay iyadoo la adeegsanayo tiir C18 leh acetonitrile iyo 10% triethylamine-acetic acid (TEAA) sida eluent.Ka dib marka la dhammeeyo dhamaystirka, waa la xoojiyaa, ka dibna kooxda DMT waxaa laga saaraa trifluoroacetic acid.Dhexdhexaadinta ka dib, milix iyo unugyo yaryar ayaa laga soo saarayaa tuubo la gooyay, oo ugu dambeyntii la macmalay.
Habkani wuxuu heli karaa badeecad leh nadiif sare, laakiin waa lagama maarmaan in fiiro gaar ah loo yeesho dhacdooyinka depurination.
3. DMT-off, HPLC Nadiifinta
Dooro DMT-Off inta lagu guda jiro isku-dhafka, iyo badeecada ceyriinka ah ayaa la xoojiyay oo lagu ururiyay heerkulka qolka si meesha looga saaro ammonia xad-dhaaf ah ka dib ammonolysis.
Kala-soocidda waxaa lagu sameeyay iyadoo la adeegsanayo tiir C18 leh acetonitrile iyo 10% triethylamine-acetic acid ee biyaha sida eluent.Ka dib marka la dhammeeyo kala-soocidda oo la qiyaaso, aliquots waa lyophilized.
Habkani wuxuu u baahan yahay in si taxadar leh loo hagaajiyo xaaladaha kala soocida, wuxuuna sidoo kale heli karaa unugyo bartilmaameed oo saafi ah.
