Mabda'a Oligo Synthesizer
Dhinacyada bayooloojiyada molecular iyo cilmi-baarista hidde-sideyaasha, awoodda lagu soo saari karo DNA ayaa door muhiim ah ka ciyaartaa.Isku-dubarid DNA waxay ku lug leedahay wax-soo-saarka macmalka ah ee molecules DNA-ga iyadoo loo habeeyey nucleotide si gaar ah.Si taas loo gaaro, saynisyahannadu waxay ku tiirsan yihiin qalab awood leh oo loo yaqaanno oligonucleotide synthesizer, oo sidoo kale loo yaqaanno DNA synthesizer.
Oligonucleotide synthesizer waa qalab casri ah oo si toos ah u soo saara molecules DNA-ga gaaban ee loo yaqaan oligonucleotides.Qaybahan gaagaaban ee DNA-da ayaa caadi ahaan dhererkoodu u dhexeeyaa 10 ilaa 100 nucleotide waana xidhidh dhismeed lama huraan u ah codsiyo kala duwan oo ay ka mid yihiin falcelinta silsilada polymerase (PCR), synthesis gene, engineering genetic, iyo sequencing DNA.
Oligonucleotide synthesizers waxay ku shaqeeyaan mabda'a farsamada loo yaqaansuunka adag.Habkan waxaa markii ugu horreysay hormuud ka ahaa Abaalmarinta Nobel ee Dr. Marvin Caruthers sannadihii 1970-aadkii, waxaana la sifeeyay sannadihii la soo dhaafay si kor loogu qaado isku-dhafka taxanaha DNA-da.Isku-dubaridka Oligonucleotide waxaa lagu fuliyaa si tartiib tartiib ah oo loogu daro hadhaaga nucleotide ilaa 5'-terminus ee silsiladda sii kordheysa ilaa inta la rabo la isu keeno.Isku darka kasta waxaa loo tixraacaa sida wareegga synthesis waxayna ka kooban tahay afar falcelin kiimikaad:
Talaabada 1: Joojinta xannibaadda ------Tallaabada 2: Isku-xidhka-----Talaabada 3: Soo koobid--------Tallaabada 4: Oxidation
Nidaamkan waxaa lagu soo celiyaa nucleotide kasta ilaa inta la helayo isku xigxiga la rabo.Muddo dheer oligonucleotides, wareeggan ayaa laga yaabaa inuu u baahdo in lagu celiyo dhowr jeer si loo soo saaro dhammaan taxanaha. Awoodda si sax ah loo xakameeyo tallaabo kasta oo ka mid ah wareegga isku-dhafka ayaa muhiim u ah oligonucleotide synthesizer.Reagen-yaasha la isticmaalo, sida nucleotides iyo activators, waxay u baahan yihiin inay ahaadaan kuwo tayo sare leh si loo xaqiijiyo isku dhafka saxda ah oo hufan.Intaa waxaa dheer, synthesizers waxay u baahan yihiin xakamaynta heerkulka saxda ah ee saxda ah iyo xaaladaha kale ee deegaanka si kor loogu qaado falcelinta isku-xidhka ee la rabo oo looga hortago falcelinta aan loo baahnayn.
Marka oligonucleotide si buuxda loo farsameeyo, waxaa sida caadiga ah laga gooyaa taageerada adag oo la nadiifiyaa si looga saaro wax kasta oo ka haray kooxaha ilaalinta ama wasakhda.Oligonucleotides-ka la safeeyey ayaa markaa diyaar u ah codsiyada hoose.
Horumarka tignoolajiyada ayaa suurtageliyay horumarinta oligonucleotide synthesizers-sare oo awood u leh inay isku mar isku mariyaan boqollaal ama xitaa kumanaan oligonucleotides.Qalabyadani waxay adeegsadaan tignoolajiyada isku dhafka ah ee ku salaysan microarray, taasoo awood u siinaya cilmi-baarayaasha inay si degdeg ah u soo saaraan maktabado oligonucleotide oo waaweyn ujeedooyin cilmi-baaris oo kala duwan.
Marka la soo koobo, mabaadi'da ka dambeysa oligonucleotide synthesizers waxay ku wareegsan yihiin farsamooyinka isku-dhafka ah ee wejiga adag, taas oo ku lug leh ku-darka nucleotides ee taageerada adag.Xakamaynta saxda ah ee wareegga isdhexgalka iyo reagents tayo sare leh ayaa lagama maarmaan u ah isku dhafka saxda ah oo hufan.Soosaarayaasha Oligo waxay door muhiim ah ka ciyaaraan cilmi-baarista DNA-da, taas oo awood u siinaya saynisyahano inay soo saaraan oligonucleotides-ka caadiga ah ee codsiyada kala duwan, oo gacan ka geysta horumarinta cilmiga bayoolajiga iyo cilmi-baarista hiddaha.
Waqtiga boostada: Agoosto-01-2023