DNA-da caadiga ah, RNA iyo isku-dhafka asiidhyada nucleic-ka ee aan-dabiiciga ahayn, tallabada Ilaalinta iyo Isku-xidhka ayaa ka ciyaarta door muhiim ah.
Talaabada difaaciddu waa in laga saaro kooxda DMT ee taageerada adag ama kooxda 5' hydroxyl ee nukleoside hore oo leh aashitada organic, oo ay soo bandhigto kooxda hydroxyl talaabada isku dhafka ee soo socota.3% trichloroacetic acid ee ku jira dichloromethane ama toluene ayaa inta badan loo isticmaalaa si loo fuliyo tallaabada ilaalinta.Isku-duubnaanta trichloroacetic acid iyo wakhtiga ilaalinta (waqtiga xannibaadda) ayaa xukuma nadiifnimada alaabta ugu dambeysa.Feejignaanta hooseeya iyo wakhtiga xannibaadda oo aan ku filnayn waxay ka tagtaa kooxda DMT ee aan la falgelin, taas oo hoos u dhigta wax-soo-saarka oo kordhiya wasakhda aan loo baahnayn.Waqtiga dheer ee xannibaadda ayaa laga yaabaa inay horseeddo depurine ee taxanaha isku dhafan, sameynta wasakh aan la filayn.
Tallaabada Isku-xidhka ayaa xasaasi u ah waxa ku jira biyaha ee dareerayaasha iyo qoyaanka hawada.Isku-duubnaanta biyaha ee isku-dhafka waa in ay ka yar tahay 40 ppm, oo ka fiican in ka yar 25 ppm.Si loo ilaaliyo xaaladda isku-dhafka anhydrous, isku-darka asiidhka nucleic-ka waa in lagu fuliyaa jawi qoyaan yar, sidaas darteed waxaan kugula talineynaa macaamiishayada inay isticmaalaanQalab Kala Diray Amidites, kaas oo ku milmi kara budada ama saliidda Fosfooraska leh ee acetonitrile anhydrous ah si looga fogaado xidhiidhka hawada.
Tan iyo markii ay milmeen fosfooraska, waxay ku fiican tahay xaaladda aan biyaha ahayn, iyo dabinada molecular si ay u dhajiyaan biyaha raadraaca ee reagents iyo amidite, waxaa loo baahan yahay in la diyaariyoDabinnada molecular.Waxaan kugula talineynaa 2 g subsieve ee dhalooyinka 50-250ml, 5g ee 250-500ml dhalooyinka reagent, 10g ee 500-1000ml dhalooyinka reagent, 20g ee 1000-2000ml dhalooyinka reagent.
Kala dirida phosphoramidites waa in lagu fuliyaa jawiga aan firfircoonayn, iyo beddelka reagents-ka firfircoonida iyo acetonitrile waa in lagu dhammeeyaa waqtiga.Reagents-ka Capping iyo Oxidation waa in sida ugu dhaqsaha badan loo isticmaalo, reagen-yaasha la furay waxay siinayaan nolol shelf yar, iyo dhaqdhaqaaq yar inta lagu jiro isku-dhafka.
Waqtiga boostada: Agoosto-09-2022