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Dabinnada molecular ee fosfooraska iyo reagents

Codsiga:

Dabinka Molecular waxaa loo isticmaalaa in lagu dhufto biyaha raadraaca reagents iyo amiite, waxaa markii hore loogu talagalay isku dhafka oligonucleotides.Waa ku habboon tahay, boodh ma laha, oo aan lahayn flannel.Waxaa lagu dari karaa noocyo kala duwan oo dareere ah iyo xalal dabiici ah si meesha looga saaro qiyaasta biyaha.

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Sharaxaada Alaabta

ABI iyo MilliGen/PerSeptive DNA synthesis tests, xidhmada shaandho-hoosaadka waxay ku hayn kartaa biyaha ku jira nitrile iyo dhalooyinka firfircoonida ka hooseeya 10 ppm, iyada oo aan laga walwalin valve ama tuubada ceejinta in loo qaybiyo shaandho-hoosaad.Boodhka ama suufka ku yaal bacda baakadaha ayaa xidhan.Waxaa si toos ah loogu isticmaali karaa nadiifinta 500 ml, 1L, 2 L iyo dhalooyinka kale ee dareeraha ah, iyo qeexida xirmooyinka shaandho-hoosaadka sidoo kale waa la habeyn karaa.

Kuwa soo socdaa waxay muujinayaan saamaynta dhaq-dhaqaaqa dareeraha biyaha ee 10 g dabinka molecular ee 500ml, 1L, 4L muunado nitrile ah oo leh biyo kala duwan.

Is barbar dhig dabinka molecular soo dejinta

500 ml 197 ppm ACN
Waqtiga (h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Wadamada kale	197	43	27	15	15

1 L 143 ppm ACN
Waqtiga (h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Wadamada kale	142	47	36	23	15

4 L 141 ppm ACN
Waqtiga (h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Wadamada kale	141	96	95	85	72

Tilmaamaha iyo isticmaalka

Dabinka molecular waa mid faaruq ah, wuxuuna u baahan yahay in la furo xitaa haddii la isticmaalo, iyo shaabadda dhalada reagent waa in la hubiyaa marka la isticmaalayo.
2g shaandho ah oo loogu talagalay 24 saac waxay yarayn kartaa 165 ppm biyaha ku jira 500 ml nitrile ilaa 105 ppm.
5 g oo shaandho ah 24 saac waxay yarayn kartaa 172 ppm biyaha ku jira 500 ml nitrile ilaa 58 ppm.
10 g shaandho 24 h Waxay yarayn kartaa 166 ppm biyaha ku jira 1 l nitrile ilaa 68 ppm.
20 g shaandho-hoosaadku waxay yarayn kartaa 162 ppm biyaha ku jira 4 L nitrile ilaa 109 ppm 24 saac.

Waxaan kugula talineynaa 2 g subsieve ee dhalooyinka 50-250ml, 5g ee 250-500ml dhalooyinka reagent, 10g ee 500-1000ml dhalooyinka reagent, 20g ee 1000-2000ml dhalooyinka reagent.

Hubinta

Xirmo kasta oo shaashad-hoosaad ah ayaa marta xakamaynta tayada adag inta lagu jiro habka wax soo saarka.Oo waa lagama maarmaan in macaamiishu ay hubiyaan baakadaha ka hor intaan la isticmaalin:

Marka hore, xaqiiji in faakuumka baakadu aanu sax ahayn.Wax kasta oo faakuum ah oo soo daadanaya ama gelitaanka hawadu waxay yaraynaysaa waxqabadka alaabta.

Marka labaad, ka taxdar markaad furto baakadda si aad uga hortagto in filimka baakad-hoosaadka ah la xoqo, oo hubi in filimku jiro daloolo.

Daaweynta ka dib isticmaalka

Ma aha sun ama waxyeello, waxay ku wasakhaysan doonaan kiimikooyinka iyo dareerayaasha ay la kulmaan waana in loo daadiyaa sida wasakhaysan isticmaalka ka dib.

Qaabka iyo codsiga

Waxaan hore u bixinay bacaha shaandho-hoosaadka oo cabbirkoodu ahaa 2 g, 5 g, 10 g, iyo 20 g., iyo tilmaamo kale ayaa loo habeyn karaa iyadoo loo eegayo baahiyaha macaamiisha

Dabinka molecular wuxuu ku habboon yahay soo-gaadhista muddada-dheer ee nitrile, waxaana sidoo kale loo isticmaali karaa acetic acid, ether, acetic ether, butyl acetate, khamriga, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid , sulfuric acid, chloride methyl, nitrogen methyl imidazole, iwm.

Waxaa laga yaabaa inaysan ku habboonayn soo-gaadhista muddada-dheer ee tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) iyo xalal kale.